Samarkand or Samarqand ( SAM-ər-kand; Uzbek: Самарқанд, romanized: Samarqand, pronounced [sæmærqænd, -ænt]; Tajik: Самарқанд; Persian: سمرقند; Sogdian: *smā́rkąθ, 𐼑𐼍𐼀𐼘𐼋𐼎𐼌𐼆‎, 𐼼𐼺𐼰𐽀𐼸𐼻𐼹𐼳‎ smʾrknδH) is a city in southeastern Uzbekistan and among the oldest continuously inhabited cities in Central Asia. There is evidence of human activity in the area of the city from the late Paleolithic Era. Though there is no direct evidence of when Samarkand was founded, several theories propose that it was founded between the 8th and 7th centuries BCE. Prospering from its location on the Silk Road between China, Persia and Europe, at times Samarkand was one of the largest cities of Central Asia. Most of the inhabitants of the city are native speakers of the Tajik dialect of the Persian language. Samarkand is one of the historical centers of the Tajik people in Central Asia, and in the past was an important city of the great empires of Greater Iran.

By the time of the Persian Achaemenid Empire, it was the capital of the Sogdian satrapy. The city was conquered by Alexander the Great in 329 BCE, when it was known as Markanda, which was rendered in Greek as Μαράκανδα. The city was ruled by a succession of Iranian and Turkic rulers until it was conquered by the Mongols under Genghis Khan in 1220. Today, Samarkand is the capital of Samarqand Region and a district-level city, that includes the urban-type settlements Kimyogarlar, Farxod and Xishrav. With 551,700 inhabitants (2021), it is the second-largest city of Uzbekistan.

The city is noted as a centre of Islamic scholarly study and the birthplace of the Timurid Renaissance. In the 14th century, Timur (Tamerlane) made it the capital of his empire and the site of his mausoleum, the Gur-e Amir. The Bibi-Khanym Mosque, rebuilt during the Soviet era, remains one of the city's most notable landmarks. Samarkand's Registan square was the city's ancient centre and is bounded by three monumental religious buildings. The city has carefully preserved the traditions of ancient crafts: embroidery, goldwork, silk weaving, copper engraving, ceramics, wood carving, and wood painting. In 2001, UNESCO added the city to its World Heritage List as Samarkand – Crossroads of Cultures.

Modern Samarkand is divided into two parts: the old city, and the new city, which was developed during the days of the Russian Empire and Soviet Union. The old city includes historical monuments, shops, and old private houses; the new city includes administrative buildings along with cultural centres and educational institutions. On September 15–16, 2022, the city hosted the 2022 SCO summit.