A twin-lens reflex camera (TLR) is a type of camera with two objective lenses of the same focal length. One of the lenses is the photographic objective or "taking lens" (the lens that takes the picture), while the other is used for the viewfinder system, which is usually viewed from above at waist level.

In addition to the objective, the viewfinder consists of a 45-degree mirror (the reason for the word reflex in the name), a matte focusing screen at the top of the camera, and a pop-up hood surrounding it. The two objectives are connected, so that the focus shown on the focusing screen will be exactly the same as on the film. However, many inexpensive 'pseudo' TLRs are fixed-focus models. Most TLRs use leaf shutters with shutter speeds up to 1/500 of a second with a bulb setting.

For practical purposes, all TLRs are film cameras, most often using 120 film, although there are many examples which used 620 film, 127 film, and 35 mm film. Few general-purpose digital TLR cameras exist, since the heyday of TLR cameras ended long before the era of digital cameras. In 2015, MiNT Camera released Instantflex TL70, a twin-lens reflex camera that uses Fuji instax mini film.