AFI may refer to:

• Address-family identifier, a 16 bit field of the Routing Information Protocol
• Ashton Fletcher Irwin, an Australian drummer
• AFI (band), an American rock band
  • AFI (2004 album), a retrospective album by AFI released in 2004
  • AFI (2017 album), the tenth studio album by AFI
• Afi, Iran, a village in East Azerbaijan Province, Iran
• Agencia Federal de Investigación (Federal Investigations Agency), a Mexican agency
• Air Force Instruction, documented instructions for members of the United States Air Force
• Akrukay language
• Alliance for Financial Inclusion, an organization of central bank regulators from the developing world
• American Film Institute, an independent non-profit film organization
• American Football Israel, a nonprofit sports organization
• Amniotic fluid index, a measure of the amount of amniotic fluid of a fetus
• Aniridia Foundation International, a support organization for people with Aniridia and family members
• Application Family Identifier, an 8 bit field of an RFID tag
• Aquarium Fish International, a monthly magazine
• Armed Forces Insurance, insurance company for American military professionals
• Association des Femmes Ivoiriennes, women's organization in the Ivory Coast
• Australian Film Institute, an organisation that promotes Australian film and television
• Nikon AF-I, a type of Nikon F-mount lens
• Agencia Federal de Inteligencia, the Argentine national intelligence agency
• Actual flip-angle imaging, a type of Magnetic resonance imaging(MRI)