Gel electrophoresis - meaning and definition. What is Gel electrophoresis
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What (who) is Gel electrophoresis - definition

METHOD FOR SEPARATION AND ANALYSIS OF MACROMOLECULES
Denaturing gel electrophoresis; Gel electrophoreisis; Native gel electrophoresis; Electrophoresis gel; Gel Electrophoresis; Native gel; Native Gel Electrophoresis; Denaturing gel; Electrophoresis device; Gel box; Helena Laboratories; Helena Laboratories Corporation; Starch gel electrophoresis
  • Gel electrophoresis is a process where an electric current is applied to DNA samples creating fragments that can be used for comparison between DNA samples.
<ol>
<li> DNA is extracted. </li>
<li> Isolation and amplification of DNA.  </li>
<li> DNA added to the gel wells.  </li>
<li> Electric current applied to the gel. </li>
<li> DNA bands are separated by size. </li>
<li> DNA bands are stained.  </li>
</ol>
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  • '''SDS-PAGE [[autoradiography]]''' – The indicated proteins are present in different concentrations in the two samples.

Sol–gel process         
  • Simplified representation of the condensation induced by hydrolysis of TEOS
  • Schematic representation of the different stages and routes of the sol–gel technology
CONDENSATION OF MONOMERS OR OLIGOMERS DISPERSED IN A COLLOIDAL SOLUTION (SOL) INTO A BIPHASIC AQUEOUS POLYMERIC NETWORK (GEL)
Sol/gel; Sol-gel process; Sol gel; Sol-Gel; Sol-gel processing; Sol–gel; Sol-gel
In materials science, the sol–gel process is a method for producing solid materials from small molecules. The method is used for the fabrication of metal oxides, especially the oxides of silicon (Si) and titanium (Ti).
Gel blaster         
  • Two gel ball blasters. The top one is based on a [[Heckler & Koch HK416]] while the below is based on a [[Remington ACR]].
TOY GUN THAT FIRES SOFT POLYMER BEADS
Gel blasters; Gel ball shooter; Gel Blasters; Gel ball gun
A gel blaster, also known as a gel gun, gel shooter, gel marker, hydro marker, hydro blaster, water bead blaster or gelsoft, is a toy gun similar in design to airsoft guns, but the projectiles they shoot are superabsorbent polymer water beads (most commonly sodium polyacrylate, colloquially called water beads, hydrogel balls, gel balls, water bullets or simply gels), which are often sold commercially as moisture retainers for gardening and pot/vase floriculture.
Agarose gel electrophoresis         
  • Video showing assembly of the rig and loading/running of the gel.
  • Cutting out agarose gel slices. Protective equipment must be worn when using UV transilluminator.
  • Agarose gel slab in electrophoresis tank with bands of dyes indicating progress of the electrophoresis. The DNA moves towards anode.
  • Loading DNA samples into the wells of an agarose gel using a multi-channel pipette.
  • Gels of plasmid preparations usually show a major band of supercoiled DNA with other fainter bands in the same lane. Note that by convention DNA gel is displayed with smaller DNA fragments nearer to the bottom of the gel. This is because historically DNA gels were run vertically and the smaller DNA fragments move downwards faster.
  • An agarose gel cast in tray, to be used for gel electrophoresis
PHYSICOANALYTICAL TECHNIQUE
Agarose gel; Electrophoresis, agar gel; Agar gel electrophoresis

Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.

Agarose gel is easy to cast, has relatively fewer charged groups, and is particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7–2% dissolved in a suitable electrophoresis buffer.

Wikipedia

Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.

Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by the charge in agarose because the pores of the gel are too small to sieve proteins. Gel electrophoresis can also be used for the separation of nanoparticles.

Gel electrophoresis uses a gel as an anticonvective medium or sieving medium during electrophoresis, the movement of a charged particle in an electrical current. Gels suppress the thermal convection caused by the application of the electric field, and can also act as a sieving medium, slowing the passage of molecules; gels can also simply serve to maintain the finished separation so that a post electrophoresis stain can be applied. DNA gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via polymerase chain reaction (PCR), but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.