Laemmli electrophoretic technique (english) - meaning, definition, translation, pronunciation

Part of Speech

Noun Phrase

Phonetic Transcription

/læɛmli ɪˌlɛk.trəˈfɔːrətɪk tɛkˈniːk/

Meaning and Usage

The Laemmli electrophoretic technique, developed by Uwe Laemmli in 1970, is a method used primarily in biochemistry and molecular biology for the separation of proteins based on their size. This technique employs discontinuous polyacrylamide gel electrophoresis (PAGE) to resolve proteins under denaturing conditions. It is widely utilized in laboratories for its effectiveness in protein analysis, particularly in assessing the purity of proteins and the presence of specific proteins in complex mixtures.

• Frequency of Use: This term is commonly used in scientific literature and academic contexts, particularly in biochemistry and life sciences. It is less frequently used in everyday conversation.
• Oral vs. Written Context: The term is primarily found in written scientific texts, research papers, and laboratory manuals, although it may occasionally be mentioned in presentations or discussions among professionals in the field.

Example Sentences

1. The Laemmli electrophoretic technique is essential for evaluating protein samples in our experiments.

2. La técnica electroforética de Laemmli es esencial para evaluar muestras de proteínas en nuestros experimentos.

3. In today’s lab session, we will apply the Laemmli electrophoretic technique to analyze the protein composition of the cell extract.

4. En la sesión de laboratorio de hoy, aplicaremos la técnica electroforética de Laemmli para analizar la composición de proteínas del extracto celular.

5. Researchers often rely on the Laemmli electrophoretic technique for precise separation of protein complexes.

6. Los investigadores a menudo confían en la técnica electroforética de Laemmli para la separación precisa de complejos proteicos.

Idiomatic Expressions

The term "Laemmli electrophoretic technique" is quite specialized and does not typically feature in common idiomatic expressions. However, it's possible to construct context-related expressions involving "technique." Here are a few creatively constructed examples:

1. To master a technique: It's important for biochemists to master the Laemmli electrophoretic technique to ensure accurate results.

2. Es importante que los bioquímicos dominen la técnica electroforética de Laemmli para asegurar resultados precisos.

3. A technique of choice: For many researchers, the Laemmli electrophoretic technique remains the technique of choice for analyzing protein samples.

4. Para muchos investigadores, la técnica electroforética de Laemmli sigue siendo la técnica preferida para analizar muestras de proteínas.

5. Refining our technique: By refining our skills in the Laemmli electrophoretic technique, we can improve the resolution of our protein gels.

6. Al perfeccionar nuestras habilidades en la técnica electroforética de Laemmli, podemos mejorar la resolución de nuestros geles de proteínas.

Etymology

The term "Laemmli" is derived from the surname of Uwe Laemmli, the scientist who introduced the technique in his seminal 1970 paper. The word "electrophoretic" comes from "electrophoresis," which itself is composed of the Greek roots "electro-" meaning "electric" and "phoresis" meaning "carrying" or "transport." Lastly, "technique" comes from the Greek "techne," meaning "skill" or "art."

Synonyms and Antonyms

Synonyms

While there are no direct synonyms for the term due to its specificity, related terms in the context of protein analysis may include: - Polyacrylamide gel electrophoresis (PAGE) - Gel electrophoresis

Antonyms

There are no direct antonyms for this technical term, but it can be contrasted with non-separation techniques in protein analysis, such as: - Spectrophotometry (which does not separate proteins but rather measures their absorbance).