The 2000 season was the St. Louis Rams' 63rd in the National Football League (NFL) and their sixth in St. Louis. For the first time in franchise history, the Rams entered the season as the defending Super Bowl champions. The Rams finished the regular-season with a record of 10–6 but would go on to lose to the New Orleans Saints in the wild-card round of the playoffs. They led the NFL in scoring for a second straight year with 540 points. The Rams became the first team in NFL history to score more than 500 points on offense, while allowing more than 450 points on defense.

Running back Marshall Faulk was named the MVP of the regular season. It was the second straight time a Rams player was named MVP.

After the resignation of Dick Vermeil, who had been the Rams' head coach through St. Louis' 1999 championship season, Mike Martz took over as head coach, and attempted to defend the Rams' Super Bowl XXXIV title. The Rams' "Greatest Show on Turf" continued its offensive dominance, scoring 33.7 points per game.

Statistically, Football Outsiders calculates that the 2000 Rams had the most efficient rushing attack of any single-season NFL team from 1993–2010. The 2000 Rams are one of only three teams in NFL history to score 35 points or more nine times in a single season. The Denver Broncos did it 10 times in 2013. The Rams' offense offset the team's defensive struggles: St. Louis' 471 points allowed in 2000 is the most ever surrendered by an NFL team with a winning record. The Rams had the best offense in the league, but had the worst defense in the league.

The season saw the Rams change their logo and add a new color scheme of navy and gold, replacing blue and yellow, donning new uniforms in the process. The Rams' new logo consisted of a charging blue ram outlined in gold with a matching gold outline. It would be their logo for 16 years.