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Qu'est-ce (qui) est PCR$505072$ - définition

TYPE OF PCR

Touchdown PCR; Touchdown pcr; Touch-down pcr

$Scheme of touchdown PCR. The first annealing between primer and DNA occurs at the initial temperature, the second after the temperature has been lowered by <math>\Delta T</math>. Therefore, the product of the second annealing is disadvantaged by <math>2^{-1}</math>.$

polymerase chain reaction

IN VITRO METHOD FOR PRODUCING LARGE AMOUNTS OF SPECIFIC DNA OR RNA FRAGMENTS FROM SMALL AMOUNTS OF SHORT OLIGONUCLEOTIDE PRIMERS

Polymerase Chain Reaction; PCR oil; PCR amplification; Polymerase chain; Hot-start; PCR reaction; Molecular Xeroxing; Polymerase chain reacton; Applications of PCR; Mechanism of PCR; Examples of PCR; Nucleic Acid Amplification; Pcr; Applications of pcr; P.C.R.; Nucleic acid amplification; Pcr test; Single Specific Primer-PCR; SSP-PCR; PCR test; Polymerase chain reaction test; PCR testing

¦ noun Biochemistry a method of making multiple copies of a DNA sequence, involving repeated reactions with a polymerase.

Polymerase chain reaction optimization

METHODS TO REDUCE FAILURE AND INCREASE THE RELIABILITY OF PCR

PCR optimization; Pcr optimization; PCR optimisation; Polymerase chain reaction optimisation; Pcr optimisation; PCR optimising; Polymerase chain reaction optimising; Pcr optimising; PCR optimizing; Polymerase chain reaction optimizing; Pcr optimizing

The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have been developed by molecular biologists to improve PCR performance and minimize failure.

https://en.wikipedia.org/wiki/Polymerase_chain_reaction_optimization

Digital polymerase chain reaction

Digital PCR; DPCR; Droplet Digital PCR

Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA, or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR, though also more prone to error in the hands of inexperienced users.

https://en.wikipedia.org/wiki/Digital_polymerase_chain_reaction

Wikipédia

Touchdown polymerase chain reaction

The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying nonspecific sequences. The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just above this point, only very specific base pairing between the primer and the template will occur. At lower temperatures, the primers bind less specifically. Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in early steps of amplification will "swamp out" any specific sequences because of the exponential nature of polymerase amplification.

https://en.wikipedia.org/wiki/Touchdown polymerase chain reaction