Pruning is a horticultural, arboricultural, and silvicultural practice involving the selective removal of certain parts of a plant, such as branches, buds, or roots.

The practice entails the targeted removal of diseased, damaged, dead, non-productive, structurally unsound, or otherwise unwanted plant material from crop and landscape plants. Some try to remember the categories as "the 4 D's": the last general category being "deranged". In general, the smaller the branch that is cut, the easier it is for a woody plant to compartmentalize the wound and thus limit the potential for pathogen intrusion and decay. It is therefore preferable to make any necessary formative structural pruning cuts to young plants, rather than removing large, poorly placed branches from mature plants.

In nature, meteorological conditions such as wind, ice and snow, and salinity can cause plants to self-prune. This natural shedding is called abscission.

Specialized pruning practices may be applied to certain plants, such as roses, fruit trees, and grapevines. It is important when pruning that the tree's limbs are kept intact, as this is what helps the tree stay upright. Different pruning techniques may be deployed on herbaceous plants than those used on perennial woody plants. Hedges, by design, are usually (but not exclusively) maintained by hedge trimming, rather than by pruning.

Reasons to prune plants include deadwood removal, shaping (by controlling or redirecting growth), improving or sustaining health, reducing risk from falling branches, preparing nursery specimens for transplanting, and both harvesting and increasing the yield or quality of flowers and fruits.