methylated DNA - definitie. Wat is methylated DNA
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Wat (wie) is methylated DNA - definitie


Methylated DNA immunoprecipitation         
  • Workflow overview of the MeDIP procedure. MeDIP procedure is followed by array-hybridization (A) or high-throughput/next generation sequencing (B)
  • Simple workflow demonstrating a typical experiment using MeDIP-seq.
PURIFICATION TECHNIQUE
Methylated dna immunoprecipitation; MeDIP; Methylated DNA
Methylated DNA immunoprecipitation (MeDIP or mDIP) is a large-scale (chromosome- or genome-wide) purification technique in molecular biology that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5-methylcytosine (5mC).
DNA methylation         
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  • '''All methylations in a prokaryote'''. In some prokaryotic organisms, all three previously known DNA methylation types are represented (N4-methylcytosine: m4C, 5-methylcytosine: m5C and N6-methyladenine: m6A). Six examples are shown here, two of which belong to the Archaea domain and four of which belong to the Bacteria domain. The information comes from Blow et al. (2016).<ref>Matthew J. Blow, Tyson A. Clark, Chris G. Daum, Adam M. Deutschbauer, Alexey Fomenkov, Roxanne Fries, Jeff Froula, Dongwan D. Kang, Rex R. Malmstrom, Richard D. Morgan, Janos Posfai, Kanwar Singh, Axel Visel, Kelly Wetmore, Zhiying Zhao, Edward M. Rubin, Jonas Korlach, Len A. Pennacchio, Richard J. Roberts: The Epigenomic Landscape of Prokaryotes. In: PLoS Genet. 12(2), Feb 2016, S. e1005854. doi:10.1371/journal.pgen.1005854. PMID 26870957. PMC 4752239</ref> In the left column are the species names of the organisms, to the right there are examples of methylated DNA motifs. The full names of the archaea and bacterial strains are according to the NCBI taxonomy: "Methanocaldococcus jannaschii DSM 2661", "Methanocorpusculum labreanum Z", "Clostridium perfringens ATCC 13127", "Geopsychrobacter electrodiphilus DSM 16401", "Rhodopseudomonas palustris CGA009" and "Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150 "
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  • Cytosine methylation then deamination to Thymine
  • Representation of a [[DNA]] molecule that is methylated. The two white spheres represent [[methyl group]]s. They are bound to two [[cytosine]] [[nucleotide]] molecules that make up the DNA sequence.
  • Possible pathways of cytosine methylation and demethylation. Abbreviations: [[S-Adenosyl-L-homocysteine]] ('''SAH'''), [[S-adenosyl-L-methionine]] ('''SAM'''), [[DNA methyltransferase]] ('''DNA MTase'''), [[Uracil-DNA glycosylase]] ('''UNG''')
  • Dynamic of DNA methylation during mouse embryonic development. E3.5-E6, etc., refer to days after fertilization. PGC: primordial germ cells
  • Typical DNA methylation landscape in mammals
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THE COVALENT TRANSFER OF A METHYL GROUP TO EITHER N-6 OF ADENINE OR C-5 OR N-4 OF CYTOSINE
Dna methylation; Methylome; Methylomics; Cytosine methylation; Dam dcm strain; Dna Methylation; Gene methylation; DNA cytosine methylation; DNA adenine methylation
DNA methylation is a biological process by which methyl groups are added to the DNA molecule. Methylation can change the activity of a DNA segment without changing the sequence.
O-methylated flavonoid         
ANY FLAVONOID HAVING A METHYLATED HYDROXY GROUP
O-methylated flavonoids; Methylated flavonol; O-methylated flavone; O-methylated flavanone; O-methylated flavanonol; O-methylated Anthocyanidin; O-methylated anthocyanidin; O-methylated flavonol; O-methylated isoflavone; Methoxyflavonoids; Methoxyflavonoid; Methoxyflavone; O-methylated flavanol; PD 98059
The O-methylated flavonoids or methoxyflavonoids are flavonoids with methylations on hydroxyl groups (methoxy bonds). O-methylation has an effect on the solubility of flavonoids.