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The petrotympanic fissure (also known as the squamotympanic fissure or the glaserian fissure) is a fissure in the temporal bone that runs from the temporomandibular joint to the tympanic cavity.
The mandibular fossa is bounded, in front, by the articular tubercle; behind, by the tympanic part of the bone, which separates it from the external acoustic meatus; it is divided into two parts by a narrow slit, the petrotympanic fissure.
It opens just above and in front of the ring of bone into which the tympanic membrane is inserted; in this situation it is a mere slit about 2 mm. in length. It lodges the anterior process and anterior ligament of the malleus, and gives passage to the anterior tympanic branch of the internal maxillary artery.