PAS staining - определение. Что такое PAS staining
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Что (кто) такое PAS staining - определение

HISTOLOGICAL STAIN METHOD
Periodic acid-Shiff; PAS stain; Periodic acid-schiff reaction; Periodic acid-Schiff; Periodic acid Schiff; Pas stain; Periodic acid-Schiff reagent; Periodic acid-Schiff stain
  • Esophageal candidiasis, PAS stain
  • Gastric signet ring cell carcinoma histopathology, PAS stain
  • Liver in glycogen storage disease, PAS stain
  • [[Periodic acid]]

Periodic acid–Schiff stain         
Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring.
Staining         
  • hematoxylin and eosin (H&E)]].
  • Example of [[negative staining]]
TECHNIQUE USED TO ENHANCE CONTRAST OF SPECIMENS OBSERVED UNDER A MICROSCOPE
In vitro staining; Vital stains; In vivo staining; Histological staining; Histologic staining; Biological stain; Biological stains; Stain (biology); Staining (microscopy); Cell staining; Staining and labeling; Simple stain; Stain (microbiology); Staining (biology); Stain (microscopy); Staining dye; Staining dyes; Vital dye; Stain (histology); Amphophile; Amphophilic; Cell stain; Tingibility; Histologic stain; Microscopy stain; Tissue stain; Tissue staining; Histological stain; Hematological stain; Positive staining; Positive stains; Positive stain
·p.pr. & ·vb.n. of Stain.
French ship Pourquoi Pas? (2005)         
SHIP BUILT IN 2005
RV Pourquoi Pas?; Pourquoi-Pas ? (2005); French ship Pourquoi-pas ? (2004); Pourquoi Pas? (2005); IMO 9285548
Pourquoi Pas? () is a research vessel built in Saint-Nazaire, France by Alstom Marine for IFREMER and the French Navy.

Википедия

Periodic acid–Schiff stain

Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain.

• PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.

• Alcian blue/periodic acid–Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step.