methylated DNA - определение. Что такое methylated DNA
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  3. methylated DNA

Что (кто) такое methylated DNA - определение

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Methylated DNA immunoprecipitation         
  • Workflow overview of the MeDIP procedure. MeDIP procedure is followed by array-hybridization (A) or high-throughput/next generation sequencing (B)
  • Simple workflow demonstrating a typical experiment using MeDIP-seq.
PURIFICATION TECHNIQUE
Methylated dna immunoprecipitation; MeDIP; Methylated DNA
Methylated DNA immunoprecipitation (MeDIP or mDIP) is a large-scale (chromosome- or genome-wide) purification technique in molecular biology that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5-methylcytosine (5mC).
https://en.wikipedia.org/wiki/Methylated_DNA_immunoprecipitation
DNA methylation         
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  • '''All methylations in a prokaryote'''. In some prokaryotic organisms, all three previously known DNA methylation types are represented (N4-methylcytosine: m4C, 5-methylcytosine: m5C and N6-methyladenine: m6A). Six examples are shown here, two of which belong to the Archaea domain and four of which belong to the Bacteria domain. The information comes from Blow et al. (2016).<ref>Matthew J. Blow, Tyson A. Clark, Chris G. Daum, Adam M. Deutschbauer, Alexey Fomenkov, Roxanne Fries, Jeff Froula, Dongwan D. Kang, Rex R. Malmstrom, Richard D. Morgan, Janos Posfai, Kanwar Singh, Axel Visel, Kelly Wetmore, Zhiying Zhao, Edward M. Rubin, Jonas Korlach, Len A. Pennacchio, Richard J. Roberts: The Epigenomic Landscape of Prokaryotes. In: PLoS Genet. 12(2), Feb 2016, S. e1005854. doi:10.1371/journal.pgen.1005854. PMID 26870957. PMC 4752239</ref> In the left column are the species names of the organisms, to the right there are examples of methylated DNA motifs. The full names of the archaea and bacterial strains are according to the NCBI taxonomy: "Methanocaldococcus jannaschii DSM 2661", "Methanocorpusculum labreanum Z", "Clostridium perfringens ATCC 13127", "Geopsychrobacter electrodiphilus DSM 16401", "Rhodopseudomonas palustris CGA009" and "Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150 "
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  • Cytosine methylation then deamination to Thymine
  • Representation of a [[DNA]] molecule that is methylated. The two white spheres represent [[methyl group]]s. They are bound to two [[cytosine]] [[nucleotide]] molecules that make up the DNA sequence.
  • Possible pathways of cytosine methylation and demethylation. Abbreviations: [[S-Adenosyl-L-homocysteine]] ('''SAH'''), [[S-adenosyl-L-methionine]] ('''SAM'''), [[DNA methyltransferase]] ('''DNA MTase'''), [[Uracil-DNA glycosylase]] ('''UNG''')
  • Dynamic of DNA methylation during mouse embryonic development. E3.5-E6, etc., refer to days after fertilization. PGC: primordial germ cells
  • Typical DNA methylation landscape in mammals
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THE COVALENT TRANSFER OF A METHYL GROUP TO EITHER N-6 OF ADENINE OR C-5 OR N-4 OF CYTOSINE
Dna methylation; Methylome; Methylomics; Cytosine methylation; Dam dcm strain; Dna Methylation; Gene methylation; DNA cytosine methylation; DNA adenine methylation
DNA methylation is a biological process by which methyl groups are added to the DNA molecule. Methylation can change the activity of a DNA segment without changing the sequence.
https://en.wikipedia.org/wiki/DNA_methylation
O-methylated flavonoid         
ANY FLAVONOID HAVING A METHYLATED HYDROXY GROUP
O-methylated flavonoids; Methylated flavonol; O-methylated flavone; O-methylated flavanone; O-methylated flavanonol; O-methylated Anthocyanidin; O-methylated anthocyanidin; O-methylated flavonol; O-methylated isoflavone; Methoxyflavonoids; Methoxyflavonoid; Methoxyflavone; O-methylated flavanol; PD 98059
The O-methylated flavonoids or methoxyflavonoids are flavonoids with methylations on hydroxyl groups (methoxy bonds). O-methylation has an effect on the solubility of flavonoids.
https://en.wikipedia.org/wiki/O-methylated_flavonoid
DNA glycosylase         
  • Hydrolysis of cytosine to uracil
ENZYMES INVOLVED IN BASE EXCISION REPAIR
GO system; Dna glycosylases; Dna glycosylase; DNA glycosylases; Dna Glycosylase
DNA glycosylases are a family of enzymes involved in base excision repair, classified under EC number EC 3.2.
https://en.wikipedia.org/wiki/DNA_glycosylase
DNA bank         
STORAGE OF PHYSICAL DNA SAMPLES
Dna bank; DNA Bank
DNA banking is the secure, long term storage of an individual’s genetic material. DNA is most commonly extracted from blood, but can also be obtained from saliva and other tissues.
https://en.wikipedia.org/wiki/DNA_bank
DNA supercoil         
  • Supercoiled structure of circular DNA molecules with low writhe. The helical nature of the DNA duplex is omitted for clarity.
  • Figure showing the various conformational changes which are observed in circular DNA at different pH. At a pH of about 12 (alkaline), there is a dip in the sedimentation coefficient, followed by a relentless increase up to a pH of about 13, at which pH the structure converts into the mysterious "Form IV".
  • Stochastic, prokaryotic model of the dynamics of RNA production and transcription locking at the promoter region, due to PSB.
  • Drawing showing the difference between a circular DNA chromosome (a plasmid) with a secondary helical twist only, and one containing an additional tertiary superhelical twist superimposed on the secondary helical winding.
  • Supercoiled structure of linear DNA molecules with constrained ends. The helical nature of the DNA duplex is omitted for clarity.
  • Illustration of how cold shock affects the supercoiling state of the DNA, by blocking the activity of Gyrase. The signs ‘ − ’ and ‘+’ represent negative and positive supercoiling, respectively. Created with BioRender.com. Also shown is a stochastic model of gene expression during cold shock as a function of the global DNA supercoiling state. The transition from ON to OFF of the promoter (P) causes the locking of transcription (i.e. RNA production). When ON, the promoter can produce RNA, from which proteins can be produced. RNA and proteins are always subject to degradation or dilution due to cell division.
COMPRESSED DNA LOOP SUPERCOILED BY PROKARYOTES TO FIT WITHIN A SMALL SPACE
Supercoil; Supercoiling; Dna, circular; Supercoiling of DNA; Positive supercoiling; Twist (DNA); Writhe (DNA); Supercoiled; Superhelical DNA energetics; Surface wrapping of DNA; DNA supercoiling; Circular genome; Supercoiled DNA; Superhelical DNA; Supertwisted DNA; Plectonemic supercoil; Linking number of DNA; Negative supercoiling; Superhelical dna
DNA supercoiling refers to the amount of twist in a particular DNA strand, which determines the amount of strain on it. A given strand may be "positively supercoiled" or "negatively supercoiled" (more or less tightly wound).
https://en.wikipedia.org/wiki/DNA_supercoil
Ancient DNA         
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  • Map of human fossils with an age of at least ~40,000 years that yielded genome-wide data<ref name="zlaty"/>
DNA ISOLATED FROM ANCIENT SPECIMENS
Fossil DNA; Paleo DNA; Ancient DNA analysis; Ancient DNA revolution; Ancient genomes; Paleogenome; Palaeogenome
Ancient DNA (aDNA) is DNA isolated from ancient specimens. Due to degradation processes (including cross-linking, deamination and fragmentation) ancient DNA is more degraded in comparison with contemporary genetic material.
https://en.wikipedia.org/wiki/Ancient_DNA
DNA database         
DATABASE OF DNA PROFILES
National DNA database; National DNA Database; DNA databank; Dna databanking; DNA Database; Genetic Database; National dna database; Dna database; DNA databases; Forensic DNA database; Genetic genealogy database; Medical DNA database; DNA Index System; Genetic database
A DNA database or DNA databank is a database of DNA profiles which can be used in the analysis of genetic diseases, genetic fingerprinting for criminology, or genetic genealogy. DNA databases may be public or private, the largest ones being national DNA databases.
https://en.wikipedia.org/wiki/DNA_database
DNA profiling         
  • Sir [[Alec Jeffreys]], pioneer of DNA profiling. His discovery led to the conviction of [[Colin Pitchfork]] in 1988.<ref name="Eureka"/>
  • CBP]] chemist reads a DNA profile to determine the origin of a commodity.
  • Restriction Fragment Length Polymorphism
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TECHNIQUE USED TO IDENTIFY AN INDIVIDUAL ON THE BASIS OF THEIR DNA CHARACTERISTICS
Genetic fingerprint; DNA fingerprinting; DNA fingerprint; Forensic DNA; Forensic genetics; Tommie Lee Andrews; DNA Fingerprinting; DNA profile; DNA evidence; Fingerprinting dna; Fingerprinting DNA; Dna fingerprinting; Dna matching; DNA Analysis; DNA fingerprints; DNA Profiling; Familial searching; Genetic fingerprinting; Dna Fingerprinting; DNA identification; Genetic Fingerprinting; Familial DNA; Fake DNA evidence; DNA recognition; DNA siblingship testing; Core STR profile; DNA screening
DNA profiling (also called DNA fingerprinting) is the process of determining an individual's DNA characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding.
https://en.wikipedia.org/wiki/DNA_profiling
DNA fingerprinting         
  • Sir [[Alec Jeffreys]], pioneer of DNA profiling. His discovery led to the conviction of [[Colin Pitchfork]] in 1988.<ref name="Eureka"/>
  • CBP]] chemist reads a DNA profile to determine the origin of a commodity.
  • Restriction Fragment Length Polymorphism
  • issue=2}}</ref>
TECHNIQUE USED TO IDENTIFY AN INDIVIDUAL ON THE BASIS OF THEIR DNA CHARACTERISTICS
Genetic fingerprint; DNA fingerprinting; DNA fingerprint; Forensic DNA; Forensic genetics; Tommie Lee Andrews; DNA Fingerprinting; DNA profile; DNA evidence; Fingerprinting dna; Fingerprinting DNA; Dna fingerprinting; Dna matching; DNA Analysis; DNA fingerprints; DNA Profiling; Familial searching; Genetic fingerprinting; Dna Fingerprinting; DNA identification; Genetic Fingerprinting; Familial DNA; Fake DNA evidence; DNA recognition; DNA siblingship testing; Core STR profile; DNA screening
DNA fingerprinting is the same as genetic fingerprinting
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